INVITRO ASSAY SYSTEM FOR CHITIN SYNTHESIS IN INSECT TISSUE

被引：34

作者：

VARDANIS, A ^{[1
]}

机构：

[1] AGR CANADA DEPT, RES INST, LONDON N6A 5B7, ONTARIO, CANADA

来源：

LIFE SCIENCES | 1976年 / 19卷 / 12期

关键词：

D O I：

10.1016/0024-3205(76)90130-2

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The system described utilizes the abdominal integument of the grasshopper Melanoplus sanguinipes as the tissue and radioactive glucose, glucosamine or UDP-N-acetyl glucosamine as substrate. A micromethod is described that makes it possible to use as little as 2 .mu.l total volume of incubation. After an initial lag period, incorporation rates become linear for at least 2 h. Cell disruption leads to immediate and complete loss of activity.

引用

页码：1949 / 1956

页数：8

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