N,N'-DICYCLOHEXYLCARBODIIMIDE IS A SPECIFIC, REVERSIBLE INHIBITOR OF PROLINE-BETA-NAPHTHYLAMIDASE

被引：3

作者：

TAKAHASHI, T ^{[1
]}

TAKAHASHI, K ^{[1
]}

机构：

[1] UNIV TOKYO,FAC SCI,DEPT BIOPHYS & BIOCHEM,7-3-1 HONGO,TOKYO 113,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 168卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)91128-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

N,N′-Dicyclohexylcarbodiimide(DCCD) was found to inhibit the activity of proline-β-naphthylamidase purified from porcine intestinal mucosa. The inhibition is rapid and reversible, and it is not due to the dissociation of the enzyme subunits. The mode of the inhibition by DCCD is noncompetitive with respect to each of the two substrates tested. Ki values of DCCD for the enzyme were determined to be 1.9 μM with proline-β-naphthylamide and 12 μM for L-leucine ethyl ester. To our knowledge, this is the first time that DCCD was found to be a potent, reversible inhibitor for an enzyme. © 1990.

引用

页码：1001 / 1006

页数：6