CA-2+ RELEASE BY INOSITOL TRISPHOSPHATE FROM CA-2+-TRANSPORTING MICROSOMES DERIVED FROM UTERINE SARCOPLASMIC-RETICULUM

被引：88

作者：

CARSTEN, ME ^{[1
]}

MILLER, JD ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES, SCH MED, DEPT ANAESTHESIOL, LOS ANGELES, CA 90024 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1985年 / 130卷 / 03期

关键词：

D O I：

10.1016/0006-291X(85)91718-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Microsomes derived from [bovine] pregnant uterine sarcoplasmic reticulum, isolated by differential and sucrose density gradient centrifugation, accumulates Ca2+ in the presence of ATP. Inositol trisphosphate caused release of this Ca2+, in a dose dependent manner. Forty percent of the Ca2+ that can be released by the ionophore A23187 was released by 5 .mu.M inositol trisphosphate. Removal of Mg by EDTA prior to addition of inositol trisphosphate did not change the course of Ca2+ release. By mobilizing intracellular Ca2+, inositol trisphosphate may be the link between hormonal stimuli and smooth muscle contraction.

引用

页码：1027 / 1031

页数：5

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