SCL/TAL-1 EXPRESSION IN T-ACUTE LYMPHOBLASTIC-LEUKEMIA - AN IMMUNOHISTOCHEMICAL AND GENOTYPIC STUDY

被引：11

作者：

CHETTY, R

PULFORD, K

JONES, M

MATHIEUMAHUL, D

CLOSE, P

HUSSEIN, S

PALLESEN, G

RALFKIAER, E

STEIN, H

GATTER, K

MASON, D

机构：

[1] INST GENET MOLEC,MONTPELLIER,FRANCE

[2] UNIV CAPE TOWN,SCH MED,DEPT PATHOL ANAT,CAPE TOWN 7925,SOUTH AFRICA

[3] ROYAL MELBOURNE HOSP,DEPT DIAGNOST HAEMATOL,MELBOURNE,VIC,AUSTRALIA

[4] AARHUS UNIV,INST PATHOL,IMMUNOHISTOL LAB,DK-8000 AARHUS,DENMARK

[5] UNIV COPENHAGEN,HERLEV HOSP,DEPT PATHOL,DK-2730 HERLEV,DENMARK

[6] FREE UNIV BERLIN,INST PATHOL,BERLIN,GERMANY

来源：

HUMAN PATHOLOGY | 1995年 / 26卷 / 09期

关键词：

SCL/TAL1; T-ACUTE LYMPHOBLASTIC LEUKEMIA; IMMUNOHISTOCHEMISTRY; POLYMERASE CHAIN REACTION;

D O I：

10.1016/0046-8177(95)90089-6

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

A comparative study of the immunohistochemical (Stem cell leukemia/T-cell acute leukemia [SCL/TAL-1] protein expression) and genotypic (deletions in the SCL/tal-1 gene) findings in T-acute lymphoblastic leukemia (T-ALL) is presented. Formalin-fixed tissue from 50 cases of T-ALL were stained with a novel monoclonal antibody, 2TL 242, which recognizes SCL/TAL-1 protein. Twenty-four cases showed nuclear immunolabeling of leukemic cells. Nuclear positivity was not evident in any other type of leukemia or lymphoma tested with the antibody. Genotypic analysis of 25 cases of T-ALL showed a deletion involving the SCL/tal-1 gene in nine cases. These results suggest that protein expression is not dependent on derangement of the SCL/tal-1 gene, because immunohistochemical detection of the protein was noted in the presence and absence of a tal-dl deletion. Copyright (C) 1995 by W.B. Saunders Company

引用

页码：994 / 998

页数：5