INCREASED RESPONSIVENESS OF RAT MAST-CELLS TO COMPOUND-48/80 DUE TO REMOVAL OF EXTRACELLULAR MAGNESIUM - EFFECTS OF OUABAIN AND EGTA

被引：3

作者：

BERTELSEN, H ^{[1
]}

JOHANSEN, T ^{[1
]}

机构：

[1] ODENSE UNIV, SCH MED,INST MED BIOL,DEPT PHARMACOL, JB WINSLOWS VEJ 19, DK-5000 ODENSE, DENMARK

来源：

FEBS LETTERS | 1991年 / 283卷 / 02期

关键词：

SECRETION; HISTAMINE; MAGNESIUM; EGTA; MAST CELL; OUABAIN;

D O I：

10.1016/0014-5793(91)80584-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A decreased secretory response of mast cells to compound 48/80 (12% of control value) after preincubation of the cells with magnesium but without calcium was partially restored by removal of magnesium. EGTA (10-mu-M) blocked the restoration and decreased the restored secretory activity again, while this was further increased by ouabain (1 mM). Furthermore, ouabain completely restored the decreased secretion (50% of control value) due to preincubation without the divalent cations. This may indicate that magnesium influences a pool of cellular calcium that is involved in the stimulus-secretion coupling and is available to EGTA, and ouabain did not counteract the inhibitory mechanism of magnesium.

引用

页码：185 / 188

页数：4

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