DETERMINATION OF UREA IN BLOOD-SERUM BY A UREASE BIOSENSOR BASED ON AN ION-SENSITIVE FIELD-EFFECT TRANSISTOR

被引:50
作者
BOUBRIAK, OA
SOLDATKIN, AP
STARODUB, NF
SANDROVSKY, AK
ELSKAYA, AK
机构
[1] INST MOLEC BIOL & GENET,150 ZABOLOTNOGO ST,KIEV 252143,UKRAINE
[2] KIEV TG SHEVCHENKO STATE UNIV,KIEV 252017,UKRAINE
关键词
BIOSENSORS; BLOOD SERUM; ISFETS; UREA;
D O I
10.1016/0925-4005(94)01633-S
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A urease biosensor for the determination of urea in solutions and blood serum is made by immobilising urease in a bovine serum albumin membrane on the surface of an ion-sensitive field-effect transistor (ISFET). The sensor gives a linear response for concentrations of urea up to 2 mM at optimum parameters of the working buffer (10 mM potassium buffer, pH 7.4 with 200 mM NaCl). The high level of proteins in the serum decreases the biosensor response, but a 25-fold serum dilution gives reliable measurements. Twenty analyses can be made without a change in the sensor output. Repeated values do not differ by more than 10%. Urea measurements with the biosensor and the chemical urease-indophenol method show a high correlation. We propose that this biosensor is suitable for the medical determination of urea in blood serum.
引用
收藏
页码:429 / 431
页数:3
相关论文
共 11 条
  • [11] Weatherburn, Phenol-hypochlorite reaction for determination of ammonia, Analytical Chemistry, 39, pp. 163-166, (1967)