SCAVENGING EFFECT OF BERBAMINE ON ACTIVE OXYGEN RADICALS IN PHORBOL ESTER-STIMULATED HUMAN POLYMORPHONUCLEAR LEUKOCYTES

被引：17

作者：

JU, HS ^{[1
]}

LI, XJ ^{[1
]}

ZHAO, BL ^{[1
]}

HAN, ZW ^{[1
]}

XIN, WJ ^{[1
]}

机构：

[1] ACAD SINICA,INST BIOPHYS,BEIJING 100080,PEOPLES R CHINA

来源：

BIOCHEMICAL PHARMACOLOGY | 1990年 / 39卷 / 11期

关键词：

D O I：

10.1016/0006-2952(90)90110-7

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The scavenging effect of berbamine (Ber) on active oxygen radicals was studied, using a spin-trapping technique and a chemiluminescence (CL) method in phorbol myristate acetate (PMA) stimulated polymorphonuclear leukocytes (PMN) and in four cell-free superoxide (O-2) or hydroxyl radical (OH.) generating systems. Ber (0.1 to 0.3 mM) effectively reduced active oxygen radicals in PMN stimulated with PMA, but had no obvious effect on oxygen consumption during the respiratory burst of PMN, measured with spin probe oxymetry. Ber (0.3 mM) prominently inhibited the CL response of PMA-stimulated PMN. The agent remarkably quenched O+2; in xanthine/xanthine oxidase and irradiation riboflavin systems and OH. in the Fenton reaction. Its scavenging action on O-2 was stronger than that of Vitamin E in the xanthine/xanthine oxidase system but the same as Vitamin E in the riboflavin system, and its action on OH. was similar to that of Vitamin E. © 1990.

引用

页码：1673 / 1678

页数：6

共 20 条

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