EXPERIMENTAL INVESTIGATION OF FIBEROPTIC CONFOCAL SCANNING MICROSCOPY - INCLUDING A COMPARISON WITH PINHOLE DETECTION

被引:10
作者
GU, M
SHEPPARD, CJR
机构
[1] Department of Physical Optics, University of Sydney
关键词
CONFOCAL MICROSCOPY; OPTICAL FIBERS;
D O I
10.1016/0968-4328(93)90032-V
中图分类号
TH742 [显微镜];
学科分类号
摘要
Reported in this paper are the experimental results on fibre-optical confocal scanning microscopy (FOCSM). We have performed various measurements in the FOCSM involving axial responses, edge responses, signal levels and interference axial responses in order to demonstrate the differences in confocal scanning imaging between using optical fibres and using pinholes. Experimentally measured results are compared with our theoretical predictions, revealing good agreements. It is shown convincingly that the FOCSM is a purely coherent imaging system as predicted in our previous theory.
引用
收藏
页码:557 / 565
页数:9
相关论文
共 24 条
[1]   CONFOCAL COMPACT SCANNING OPTICAL MICROSCOPE BASED ON COMPACT-DISK TECHNOLOGY [J].
BENSCHOP, J ;
VANROSMALEN, G .
APPLIED OPTICS, 1991, 30 (10) :1179-1184
[2]   SINGLE-MODE FIBERS USED AS CONFOCAL MICROSCOPE PINHOLES [J].
DABBS, T ;
GLASS, M .
APPLIED OPTICS, 1992, 31 (06) :705-706
[3]   FIBEROPTIC CONFOCAL MICROSCOPE - FOCON [J].
DABBS, T ;
GLASS, M .
APPLIED OPTICS, 1992, 31 (16) :3030-3035
[4]   FLUORESCENCE LIFETIME MEASUREMENTS USING A NOVEL FIBEROPTIC LASER SCANNING CONFOCAL MICROSCOPE [J].
GHIGGINO, KP ;
HARRIS, MR ;
SPIZZIRRI, PG .
REVIEW OF SCIENTIFIC INSTRUMENTS, 1992, 63 (05) :2999-3002
[5]  
Goodman J, 1996, INTRO FOURIER OPTICS
[6]   IMAGE-FORMATION IN A FIBEROPTIC CONFOCAL SCANNING MICROSCOPE [J].
GU, M ;
SHEPPARD, CJR ;
GAN, X .
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 1991, 8 (11) :1755-1761
[7]  
GU M, 1993, OPTIK, V93, P87
[8]   3-DIMENSIONAL COHERENT TRANSFER-FUNCTIONS IN FIBEROPTIC CONFOCAL SCANNING MICROSCOPES [J].
GU, M ;
GAN, X ;
SHEPPARD, CJR .
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 1991, 8 (07) :1019-1025
[9]   IMAGE OF A STRAIGHT EDGE IN FIBEROPTIC CONFOCAL SCANNING MICROSCOPY [J].
GU, M ;
SHEPPARD, CJR .
OPTICS COMMUNICATIONS, 1992, 94 (06) :485-490
[10]   AXIAL RESOLUTION IN THE FIBEROPTIC CONFOCAL SCANNING MICROSCOPE USING ANNULAR LENSES [J].
GU, M ;
SHEPPARD, CJR .
OPTICS COMMUNICATIONS, 1992, 88 (01) :27-32