ONLINE RAMAN-SPECTROSCOPY OF RIBONUCLEOTIDES PRECONCENTRATED BY CAPILLARY ISOTACHOPHORESIS

被引：32

作者：

WALKER, PA ^{[1
]}

KOWALCHYK, WK ^{[1
]}

MORRIS, MD ^{[1
]}

机构：

[1] UNIV MICHIGAN,DEPT CHEM,ANN ARBOR,MI 48109

来源：

ANALYTICAL CHEMISTRY | 1995年 / 67卷 / 23期

关键词：

D O I：

10.1021/ac00119a009

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Normal Raman spectroscopy is used as an on-line detector for capillary isotachophoresis (ITP) of adenosine 5'-triphosphate, adenosine 5'-diphosphate, and adenosine 5'-monophosphate in phosphate buffers, Preconcentration is from a 1 x 10(-2) M phosphate buffer (pH 7.5) into a leading electrolyte of 0.1 M KCl or Na2SO4, with a terminating electrolyte of 0.1 M 4-morpholinepropane-sulfonic acid, The ribonucleotides are concentrated to above 10(-2) M at the detection window, allowing measurement of Raman spectra with 1 s integration, from starting concentrations of 5 x 10(-6) M or higher.

引用

页码：4255 / 4260

页数：6

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