SPATIALLY RESOLVED TEMPERATURE-MEASUREMENTS IN ELECTROPHORESIS CAPILLARIES BY RAMAN THERMOMETRY

被引：53

作者：

DAVIS, KL ^{[1
]}

LIU, KLK ^{[1
]}

LANAN, M ^{[1
]}

MORRIS, MD ^{[1
]}

机构：

[1] UNIV MICHIGAN, DEPT CHEM, ANN ARBOR, MI 48109 USA

来源：

ANALYTICAL CHEMISTRY | 1993年 / 65卷 / 03期

关键词：

D O I：

10.1021/ac00051a018

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Local temperatures inside a 75-mum-i.d. capillary under electrophoresis conditions are measured noninvasively with a Raman microprobe. The method is based on the temperature dependence of the water O-H stretch equilibrium between weakly bent and strongly bent hydrogen bonded species. With calibration against a known temperature standard, this technique is shown to be capable of obtaining spatially resolved intracapillary temperature measurements with an accuracy of +/-1.0-degrees-C and a precision of +/-0.1-degrees-C. Intracapillary temperatures ranging from 25 to 70-degrees-C are observed over the range of buffer compositions and electric fields used. Differences between local temperatures and average capillary temperatures are observed at all operating conditions. The difficulty of an accurate theoretical description of heal dissipation under CE conditions is discussed.

引用

页码：293 / 298

页数：6

共 40 条

[1] MICRO-RAMAN SPECTROSCOPY FOR CHARACTERIZATION OF SEMICONDUCTOR-DEVICES [J].