Engineering the haploid genome of microspores

被引：9

作者：

Francois, Eudes ^{[1
]}

Youn-Seb, Shim ^{[2
]}

Jiang Fengying ^{[1
]}

机构：

[1] Agr & Agri Food Canada, 5403 1st Ave South, Lethbridge, AB T1J 4B1, Canada

[2] Nunhems USA Inc, Davis, CA 95618 USA

来源：

BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY | 2014年 / 3卷 / 01期

关键词：

Doubled haploid; Transfection; Plant transformation; Gene delivery method;

D O I：

10.1016/j.bcab.2013.11.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Microspore culture is not only a valuable system for the study of developmental plant biology, but can also be effectively exploited for biotechnological purposes. Microspore culture is an established platform for the production of large numbers of doubled haploid plants in a few crop species. The unique characteristics of this haploid cell and the rapid advancement of genome sequencing technology make the microspore a special target for genetic engineering. DNA and/or protein delivery into these cells has been studied during the past 25 years with mitigated success, and was often limited to daughter cells. A novel delivery method using short peptide nanocarriers has rendered microspore transformation more achievable and opened new opportunities for crop development. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.

引用

页码：20 / 23

页数：4

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