An efficient method for transformation of pre-androgenic, isolated Brassica napus microspores involving microprojectile bombardment and Agrobacterium-mediated transformation

被引：22

作者：

Abdollahi, M. R. ^{[2
]}

Corral-Martinez, P. ^{[1
]}

Mousavi, A. ^{[3
]}

Salmanian, A. H. ^{[3
]}

Moieni, A. ^{[2
]}

Segui-Simarro, J. M. ^{[1
]}

机构：

[1] Univ Politecn Valencia, COMAV, Valencia 46022, Spain

[2] Tarbiat Modares Univ, Fac Agr, Tehran, Iran

[3] Natl Inst Genet Engn & Biotechnol, Tehran, Iran

来源：

ACTA PHYSIOLOGIAE PLANTARUM | 2009年 / 31卷 / 06期

关键词：

Agrobacterium-mediated transformation; Agroinfiltration; Biolistics; Rapeseed; GUS expression; Microprojectile bombardment; IMMEDIATE COLCHICINE TREATMENT; DOUBLED HAPLOID PLANTS; PARTICLE BOMBARDMENT; GENE-TRANSFER; MAIZE MICROSPORES; OILSEED RAPE; EMBRYOGENESIS; EXPRESSION; EMBRYOS; INDUCTION;

D O I：

10.1007/s11738-009-0365-5

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The physical barrier imposed by the thick microspore wall constitutes an obstacle for an efficient Agrobacterium-mediated transformation of vacuolate microspores prior to androgenic induction and haploid embryogenic commitment. It is thus necessary to implement additional methods to overcome this drawback. In this study, we focused on the optimization of a protocol to allow for the exogenous DNA to enter the microspore in an efficient manner. We tested different options, based on microprojectile bombardment, to be applied prior to agroinfiltration. From them, the best results were obtained through co-transformation by microspore bombardment with DNA-coated microprojectile particles, followed by Agrobacterium tumefaciens infection. This method provides an efficient means to integrate extraneous DNA into rapeseed microspores prior to androgenesis induction.

引用

页码：1313 / 1317

页数：5

共 30 条

[1] MICROPROJECTILE BOMBARDMENT OF PLANT-TISSUES INCREASES TRANSFORMATION FREQUENCY BY AGROBACTERIUM-TUMEFACIENS [J].