CONTROL OF C-JUN ACTIVITY BY INTERACTION OF A CELL-SPECIFIC INHIBITOR WITH REGULATORY DOMAIN-DELTA - DIFFERENCES BETWEEN V-JUN AND C-JUN

被引：216

作者：

BAICHWAL, VR ^{[1
]}

TJIAN, R ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY, DEPT MOLEC & CELLULAR BIOL, BERKELEY, CA 94720 USA

来源：

CELL | 1990年 / 63卷 / 04期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0092-8674(90)90147-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Analysis of transcriptional activation properties of c-Jun chimeras in different cell lines suggests that it contains an activator domain (A1) that is negatively regulated by a cell type-specific inhibitor. A regulatory domain of c-Jun, δ, previously identified by in vitro experiments, also regulates transcriptional activation by c-Jun in vivo. The δ domain facilitates or stabilizes the interaction of the cellular inhibitor with A1. v-Jun, which lacks δ, is a stronger transcriptional activator than c-Jun, since its activity is not efficiently repressed by the cellular inhibitor. In vitro transcription with chimeric Jun proteins and extracts from different cell types confirms that the A1 and δ domains are repressed in a cell type-specific manner. These findings implicate a specific cellular factor in the negative regulation of c-Jun activity and suggest a molecular basis for the observed difference in transcriptional properties between v-Jun and c-Jun. © 1990.

引用

页码：815 / 825

页数：11

共 49 条

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