LACTOSE WHEY UTILIZATION AND ETHANOL-PRODUCTION BY TRANSFORMED SACCHAROMYCES-CEREVISIAE CELLS

被引:49
作者
PORRO, D [1 ]
MARTEGANI, E [1 ]
RANZI, BM [1 ]
ALBERGHINA, L [1 ]
机构
[1] UNIV MILAN,DIPARTIMENTO FISIOL & BIOCHIM GEN,SEZ BIOCHIM COMPARATA,I-20133 MILAN,ITALY
关键词
SACCHAROMYCES-CEREVISIAE; LACTOSE WHEY; BIOMASS AND ETHANOL PRODUCTION;
D O I
10.1002/bit.260390802
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Strains of Saccharomyces cerevisiae transformed with a multicopy expression vector bearing both the Escherichia coli beta-galactosidase gene under the control of the upstream activating sequence of the GAL1-10 genes and the GAL4 activator gene release part of beta-galactosidase in the growth medium. This release is due to cell lysis of the older mother cells; the enzyme maintains its activity in buffered growth media. Fermentation studies with transformed yeast strains showed that the release of beta-galactosidase allowed an efficient growth on buffered media containing lactose as carbon source as well as on whey-based media. The transformed strains utilized up to 95% of the lactose and a high growth yield was obtained in rich media. High productions of ethanol were also observed in stationary phase after growth in lactose minimal media.
引用
收藏
页码:799 / 805
页数:7
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