SEXING FREE-RANGING BROWN BEARS URSUS-ARCTOS USING HAIRS FOUND IN THE FIELD

被引：107

作者：

TABERLET, P

MATTOCK, H

DUBOISPAGANON, C

BOUVET, J

机构：

[1] Laboratoire de Biologie Des Populations D'altitude, Cnrs Ep55, Université Joseph Fourier, Grenoble, 38041

来源：

MOLECULAR ECOLOGY | 1993年 / 2卷 / 06期

关键词：

HAIR; POLYMERASE CHAIN REACTION; SEX IDENTIFICATION; SRY GENE; URSUS-ARCTOS; Y-CHROMOSOME;

D O I：

10.1111/j.1365-294X.1993.tb00033.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

As an aid to the management of the Pyrenean population of the brown bear Ursus arctos, a sexing method based on the amplification of a Y chromosome specific sequence has been developed, and tested using hairs found in the field as a source of DNA. This method involves a two-step polymerase chain reaction (PCR) which allows the detection of a very small amount of DNA, probably a single SRY gene molecule. The sex can reliably be identified using about 50pg of DNA extract as template. It is possible that this approach could, with adjustments, be used to identify the sex of other species of eutherian mammals.

引用

页码：399 / 403

页数：5

共 26 条

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