PEROXIDATIVE XENOBIOTIC OXIDATION BY PARTIALLY PURIFIED PEROXIDASE AND LIPOXYGENASE FROM HUMAN FETAL TISSUES AT 10 WEEKS OF GESTATION

被引：11

作者：

DATTA, K ^{[1
]}

JOSEPH, P ^{[1
]}

ROY, SK ^{[1
]}

SRINIVASAN, SN ^{[1
]}

KULKARNI, AP ^{[1
]}

机构：

[1] UNIV S FLORIDA,COLL PUBL HLTH,DEPT ENVIRONM & OCCUPAT HLTH,FLORIDA TOXICOL RES CTR,TAMPA,FL 33612

来源：

GENERAL PHARMACOLOGY | 1995年 / 26卷 / 01期

关键词：

HUMAN FETAL TISSUE; XENOBIOTIC COOXIDATION; PEROXIDASE; LIPOXYGENASE;

D O I：

10.1016/0306-3623(94)00164-I

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

1. Present study reports the ability of partially purified peroxidase and lipoxygenase from human fetal tissues at 10 weeks of gestation to oxidize selected xenobiotics in vitro. 2. Peroxidase was found to oxidize four different chemicals in the presence of H2O2. Sodium azide and potassium cyanide inhibited peroxidase activity towards guaiacol in a concentration-dependent manner. 3. The dioxygenase and co-oxidase activities of lipoxygenase towards linoleic acid and four model xenobiotics, respectively, were observed. Both the catalytic activities of lipoxygenase were significantly inhibited by <1.0 mu M nordihydroguaiaretic acid. 4. These findings suggest that peroxidase and lipoxygenase may be important pathways for peroxidative xenobiotic oxidation in human fetal tissues.

引用

页码：107 / 112

页数：6

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