FATE OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 PROVIRUS IN INFECTED-CELLS - A ROLE FOR VPR

被引：92

作者：

PLANELLES, V

BACHELERIE, F

JOWETT, JBM

HAISLIP, A

XIE, YM

BANOONI, P

MASUDA, T

CHEN, ISY

机构：

[1] UNIV CALIF LOS ANGELES,SCH MED,DEPT MICROBIOL & IMMUNOL,LOS ANGELES,CA 90094

[2] JONSSON COMPREHENS CANC CTR,LOS ANGELES,CA 90094

[3] UNIV CALIF LOS ANGELES,SCH MED,DIV HEMATOL ONCOL,LOS ANGELES,CA 90094

[4] INST PASTEUR,UNITE IMMUNOL VIRALE,F-75724 PARIS 16,FRANCE

来源：

JOURNAL OF VIROLOGY | 1995年 / 69卷 / 09期

关键词：

D O I：

10.1128/JVI.69.9.5883-5889.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We investigated the fate of human immunodeficiency virus type 1 (HIV-1) viral DNA in infected peripheral blood lymphocytes and immortalized T-cell lines by using a replication-defective HIV-1. We observed that integrated HIV-1 DNA and viral gene expression decrease over time. A frameshift mutation in vpr resulted in maintenance of the HIV-1 provirus and stable persistence of viral expression. Transfection of vpr together,vith the neomycin resistance gene in the absence of other viral genes decreased the formation of geneticin-resistant colonies, indicating either a cytotoxic or a cytostatic effect upon cells. Therefore, maintenance of HIV-1 infection within an infected proliferating population is due to two competing processes, the rate of viral spread and the degree of cell growth inhibition and/or death induced by Vpr.

引用

页码：5883 / 5889

页数：7

共 45 条

[11] MULTIFACTORIAL NATURE OF HUMAN-IMMUNODEFICIENCY-VIRUS DISEASE - IMPLICATIONS FOR THERAPY [J].