MODE OF ACTION OF FUNGICIDE DENMERT (S-1358) IN FUNGI .3. SELECTIVE-INHIBITION OF DEMETHYLATION AT C-14 IN ERGOSTEROL BIOSYNTHESIS BY FUNGICIDE, DENMERT (S-1358)

被引：53

作者：

KATO, T ^{[1
]}

KAWASE, Y ^{[1
]}

机构：

[1] SUMITOMO CHEM CO LTD, DEPT RES, DIV PESTICIDES, TAKARAZUKA, HYOGO, JAPAN

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1976年 / 40卷 / 12期

关键词：

D O I：

10.1080/00021369.1976.10862425

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

In cell-free homogenates of Saccharomyces cerevisiae, Denmert (S-1358) inhibited the incorporation of radioactivity from DL-mevalonate-2-14C into 14-desmethyl-lanosterol, 4.alpha.-methyl-cholesta-8,24-dien-3-one, 4.alpha.-methyl-zymosterol and 4-desmethyl sterols (zymosterol and episterol) at a concentration of 10-4 M. Concomitantly, a large accumulation of radioactivity was observed in the lanosterol fraction. In good agreement with results described above, Denmert inhibited the conversion of 14C-labeled lanosterol to 4-desmethyl sterols, while the conversion of 14C-labeled 14-desmethyl-lanosterol to 4-desmethyl sterols was hardly affected by the fungicide. It is evident that Denmert is a potent selective inhibitor of the demethylation at the C-14 position in ergosterol biosynthesis. The fungicide, triarimol, was found to exhibit the same effect on sterol biosynthesis as Denmert.

引用

页码：2379 / 2388

页数：10

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