EFFECTS OF GROWTH-HORMONE AND INSULIN-LIKE GROWTH FACTOR-I ON RABBIT PROXIMAL CONVOLUTED TUBULE TRANSPORT

被引:104
作者
QUIGLEY, R
BAUM, M
机构
[1] UNIV TEXAS,SW MED CTR,DEPT PEDIAT,5323 HARRY HINES BLVD,DALLAS,TX 75235
[2] UNIV TEXAS,SW MED CTR,DEPT MED,DALLAS,TX 75235
关键词
INVITRO MICROPERFUSION; PHOSPHATE TRANSPORT; BICARBONATE TRANSPORT; VOLUME ABSORPTION;
D O I
10.1172/JCI115312
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
This in vitro microperfusion study examined the effects of growth hormone and insulin-like growth factor I (IGF-I) on proximal convoluted tubule (PCT) transport. Tubules were perfused with an ultrafiltrate-like solution and bathed in a serum-like albumin solution. Neither a physiologic (5 x 10(-10) M), nor a pharmacologic (5 x 10(-8) M) dose of growth hormone had an effect on PCT phosphate or bicarbonate transport, or volume absorption. Addition of 5 x 10(-9) M and 5 x 10(-8) M IGF-I, but not 5 x 10(-10) M IGF-I, to the bathing solution resulted in an increase (12-15%) in phosphate transport, but no change in volume absorption or bicarbonate transport. Addition of IGF-I to the luminal perfusate also stimulated phosphate transport. The effect was noted at a concentration of 5 x 10(-11) M IGF-I (27% stimulation) and was maximal at a concentration of 5 x 10(-10) M IGF-I (46% stimulation). There was no effect of luminal IGF-I on volume absorption or bicarbonate transport. These data indicate that growth hormone has no direct effect on PCT transport. In the PCT, IGF-I stimulates phosphate transport specifically and acts via both basolateral and apical membranes. However, the magnitude of the maximal response to the luminal addition of IGF-I was threefold greater than that measured upon addition of the hormone to the bath, and the stimulation occurred at a 100-fold lower concentration. These data are consistent with IGF-I mediating the in vivo stimulation of phosphate transport by growth hormone.
引用
收藏
页码:368 / 374
页数:7
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[21]   DIETARY PHOSPHORUS AND 1,25-DIHYDROXYVITAMIN-D METABOLISM - INFLUENCE OF INSULIN-LIKE GROWTH FACTOR-I [J].
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[22]   REGULATION OF CANINE RENAL VESICLE PI TRANSPORT BY GROWTH-HORMONE AND PARATHYROID-HORMONE [J].
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[24]   DISTRIBUTION OF IGF RECEPTORS IN THE PLASMA-MEMBRANE OF PROXIMAL TUBULAR CELLS [J].
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[25]   BINDING OF IGF-I AND IGF-I-STIMULATED PHOSPHORYLATION IN CANINE RENAL BASOLATERAL MEMBRANES [J].
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[26]   NONSUPPRESSIBLE INSULIN-LIKE ACTIVITY (NSILA) .2. REGULATION OF SERUM CONCENTRATIONS BY GROWTH-HORMONE AND INSULIN [J].
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JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1978, 46 (04) :672-678
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