PROTON NMR-STUDY OF THE INTERACTION OF BENZO(A)PYRENE WITH RAT-LIVER MICROSOMAL CYTOCHROME-P-450

被引：5

作者：

CASTROMADERAL, L ^{[1
]}

SULLIVAN, PD ^{[1
]}

机构：

[1] OHIO UNIV,DEPT CHEM,ATHENS,OH 45701

来源：

FEBS LETTERS | 1992年 / 296卷 / 03期

关键词：

CYTOCHROME-P-450; BENZO(A)PYRENE; H-1; NMR; RELAXATION MEASUREMENT;

D O I：

10.1016/0014-5793(92)80297-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

H-1 nuclear magnetic resonance longitudinal relaxation time (T1) measurements were used to study the interaction of 1,3,6-H-benzo(a)pyrene (1,3,6-H-BaP) with microsomal cytochrome P-450 from livers of phenobarbital- and beta-naphthoflavone-treated rats. Upon addition of various amounts of cytochrome P-450 to solutions of BaP, the T1 values for the three BaP protons decreased. When the hemeprotein was converted to its carbon monoxyferrous derivative in the presence of BaP, the T1 values of the BaP protons increased. The paramagnetic contributions to the observed T1 values were calculated and resulted in distance estimates of > 6 angstrom for the three BaP protons in both cases.

引用

页码：249 / 253

页数：5