ANALYSIS OF THE OPTIMAL CONDITIONS FOR THE ADSORPTION OF TYPE-III PNEUMOCOCCAL POLYSACCHARIDE TO PLASTIC FOR USE IN SOLID-PHASE ELISA

被引：11

作者：

ELKINS, KL ^{[1
]}

STASHAK, PW ^{[1
]}

BAKER, PJ ^{[1
]}

机构：

[1] NIAID, IMMUNOGENET LAB, TWINBROOK RES FACIL 2, 12441 PARKLAWN DR, ROCKVILLE, MD 20852 USA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 130卷 / 01期

关键词：

Antibody response; ELISA; Isotype distribution; pH; Pneumococcal polysaccharide;

D O I：

10.1016/0022-1759(90)90306-G

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An economical, sensitive enzyme-linked immunosorbent assay (ELISA) method for measuring all isotypes of immunoglobulin specific for type III pneumococcal polysaccharide (SSS-III) is described, using 96-well polystyrene microtiter plates coated directly with antigen. To achieve substantial binding of SSS-III to plastic plates, the polysaccharide had to be dissolved in a buffer (0.1 M Hepes) of pH 4.0 or less. Optimal conditions for adsorption of SSS-III to plates were found to be pH 3.5 and a concentration of 1.0 μg SSS-III/ml (0.1 μg/well). Under these conditions, murine anti-SSS-III polyclonal or monoclonal antibodies could be detected to a limit of about 5-10 ng/ml. The implications of these findings for assays that use mixtures of polysaccharides adsorbed to plastic plates are discussed. © 1990.

引用

页码：123 / 131

页数：9

共 26 条

[11]

FUNG J, 1980, J IMMUNOL, V125, P1980

[12] STUDY OF THE IDIOTYPY OF LIPOPOLYSACCHARIDE-SPECIFIC POLYCLONAL AND MONOCLONAL-ANTIBODIES [J].

HIERNAUX, JR ;

SCHROER, KR ;

BAKER, PJ ;

RUDBACH, JA ;

BONA, C .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1982, 12 (10) :797-803

[13] COMPARISON OF AN ENZYME-LINKED IMMUNOSORBENT-ASSAY WITH RADIOIMMUNOASSAY FOR THE MEASUREMENT OF PNEUMOCOCCAL CAPSULAR POLYSACCHARIDE ANTIBODIES [J].

KATZ, MA ;

SCHIFFMAN, G .

MOLECULAR IMMUNOLOGY, 1985, 22 (03) :313-319

[14] COMPARISON OF ELISA AND RIA FOR MEASUREMENT OF PNEUMOCOCCAL ANTIBODIES BEFORE AND AFTER VACCINATION WITH 14-VALENT PNEUMOCOCCAL CAPSULAR POLYSACCHARIDE VACCINE [J].

KOSKELA, M ;

LEINONEN, M .

JOURNAL OF CLINICAL PATHOLOGY, 1981, 34 (01) :93-98

[15] COMBINING REGIONS OF TYPE III PNEUMOCOCCUS POLYSACCHARIDE AND HOMOLOGOUS ANTIBODY [J].

MAGE, RG ;

KABAT, EA .

BIOCHEMISTRY, 1963, 2 (06) :1278-&

[16] STANDARD SERA IN SOLID-PHASE IMMUNOASSAYS [J].

MAKELA, O ;

PETERFY, F .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1983, 13 (10) :815-819

[17] MODIFICATION OF A DIRECT ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR THE DETECTION OF IMMUNOGLOBULIN-G AND IMMUNOGLOBULIN-M ANTIBODIES TO PNEUMOCOCCAL CAPSULAR POLYSACCHARIDE [J].

MESSINA, JP ;

HICKOX, PG ;

LEPOW, ML ;

POLLARA, B ;

VENEZIA, RA .

JOURNAL OF CLINICAL MICROBIOLOGY, 1985, 21 (03) :390-394

[18] AN ENZYME-LINKED IMMUNOSORBENT-ASSAY SUITABLE FOR THE ROUTINE ESTIMATION OF SPECIFIC IMMUNOGLOBULIN-G RESPONSES TO POLYVALENT PNEUMOCOCCAL POLYSACCHARIDE VACCINE IN HUMANS [J].

NIEUWHOF, WN ;

HODGEN, AN .

JOURNAL OF IMMUNOLOGICAL METHODS, 1985, 84 (1-2) :197-202

[19] DETECTION OF ANTIBODIES TO PNEUMOCOCCAL CAPSULAR POLYSACCHARIDES BY ENZYME-LINKED IMMUNOSORBENT-ASSAY [J].

PEDERSEN, FK ;

HENRICHSEN, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 1982, 15 (03) :372-378

[20] STRUCTURE OF C-POLYSACCHARIDE FROM WALLS OF STREPTOCOCCUS-PNEUMONIAE [J].

POXTON, IR ;

TARELLI, E ;

BADDILEY, J .

BIOCHEMICAL JOURNAL, 1978, 175 (03) :1033-1042

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