RECOMBINANT SOLUBLE TUMOR-NECROSIS-FACTOR RECEPTOR PROTEINS PROTECT MICE FROM LIPOPOLYSACCHARIDE-INDUCED LETHALITY

被引：255

作者：

LESSLAUER, W ^{[1
]}

TABUCHI, H ^{[1
]}

GENTZ, R ^{[1
]}

BROCKHAUS, M ^{[1
]}

SCHLAEGER, EJ ^{[1
]}

GRAU, G ^{[1
]}

PIGUET, PF ^{[1
]}

POINTAIRE, P ^{[1
]}

VASSALLI, P ^{[1
]}

LOETSCHER, H ^{[1
]}

机构：

[1] UNIV GENEVA,CMU,DEPT PATHOL,CH-1211 GENEVA 4,SWITZERLAND

来源：

EUROPEAN JOURNAL OF IMMUNOLOGY | 1991年 / 21卷 / 11期

关键词：

D O I：

10.1002/eji.1830211134

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The in vivo efficacy of human recombinant soluble tumor necrosis factor (TNF) receptor protein to prevent and to treat lipopolysaccharide (LPS)-induced lethal toxicity in D-galactosamine-treated mice was investigated. Chimeric proteins of the receptor extracellular domains fused to the hinge region of human IgG3 were expressed in myeloma cells (rsTNFR-h-gamma-3). The fusion proteins had a disulfide-bonded dimeric structure. Upon intravenous injection, their serum concentration decreased relatively slowly after an initial phase of rapid elimination. D-galactosamine-sensitized mice were fully protected from the toxic effects of LPS, if the animal were pretreated with rsTNFR-h-gamma-3 at 20-mu-g/animal. Partial protection was seen at significantly lower doses and when rsTNFR-h-gamma-3 was given up to 3 h after LPS.

引用

页码：2883 / 2886

页数：4

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