ISOLATION OF EXFOLIATIVE TOXIN FROM STAPHYLOCOCCUS-HYICUS SUBSP HYICUS AND ITS EXFOLIATIVE ACTIVITY IN THE PIGLET

被引：40

作者：

SATO, H ^{[1
]}

TANABE, T ^{[1
]}

KURAMOTO, M ^{[1
]}

TANAKA, K ^{[1
]}

HASHIMOTO, T ^{[1
]}

SAITO, H ^{[1
]}

机构：

[1] KEIO UNIV, SCH MED, DEPT DERMATOL, SHINJUKU KU, TOKYO 160, JAPAN

来源：

VETERINARY MICROBIOLOGY | 1991年 / 27卷 / 3-4期

关键词：

D O I：

10.1016/0378-1135(91)90153-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Exfoliative toxin was isolated from the sterile cell-free filtrate of 24 h culture of Staphylococcus hyicus subsp. hyicus strain P-1. The partial purification of exfoliative toxin produced by S. hyicus (shET) was performed by precipitation with 50-80% saturated ammonium sulfate, gel filtration on a Sephadex G-75 column and column chromatography on DEAE-cellulose. Partially purified shET (pp-shET) caused exfoliation in piglets at 8 to 12 h after intradermal or subcutaneous injection. However, heat-treated pp-shET did not cause exfoliation in piglets for up to 24 h after injection. On histopathological examination of the skin at 12 h after injection of pp-shET, an intraepidermal cleavage plane was shown between the stratum corneum and stratum granulosum and at the stratum granulosum.

引用

页码：263 / 275

页数：13