REGULATION BY PROTEIN-KINASE-C OF PUTATIVE P-TYPE CA CHANNELS EXPRESSED IN XENOPUS OOCYTES FROM CEREBELLAR MESSENGER-RNA

被引：21

作者：

FOURNIER, F ^{[1
]}

CHARNET, P ^{[1
]}

BOURINET, E ^{[1
]}

VILBERT, C ^{[1
]}

MATIFAT, F ^{[1
]}

CHARPENTIER, G ^{[1
]}

NAVARRE, P ^{[1
]}

BRULE, G ^{[1
]}

MARLOT, D ^{[1
]}

机构：

[1] CTR RECH BIOCHIM MACROMOLEC, CNRS, UPR 8402, INSERM, U249, F-34033 MONTPELLIER, FRANCE

来源：

FEBS LETTERS | 1993年 / 317卷 / 1-2期

关键词：

XENOPUS OOCYTE; CEREBELLAR MESSENGER-RNA; P-TYPE CA CHANNEL; PROTEIN KINASE-C;

D O I：

10.1016/0014-5793(93)81504-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Xenopus oocytes injected with rat cerebellar mRNA expressed functional voltage-dependent Ca channels detected as an inward Ba current (I(Ba)). The pharmacological resistance to dihydropyridines and omega-conotoxin together with the blockade obtained with Agelenopsis aperta venom suggest that these channels could be somehow assimilated to P-type Ca channels. The precise nature of the transplanted Ca channels was assessed by hybrid-arrest experiments using a specific oligonucleotide antisense-derivated from the recently cloned alpha1-subunit of P channels (BI-1 clone). In addition, we demonstrate that exogenous Ca channel activity was enhanced by two different PKC activators (a phorbol ester and a structural analog to diacylglycerol). The general electrophysiological and pharmacological properties of the stimulated Ca channels remain unchanged. This potentiation induced by PKC activators is antagonized by a PKC inhibitor (staurosporine) and by a monoclonal antibody directed against PKC. It is concluded that P-type Ca channels are potentially regulated by PKC phosphorylation and the functional relevance of this intracellular pathway is discussed.

引用

页码：118 / 124

页数：7

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