C-13 NMR-SPECTROSCOPY OF KERATAN SULFATES - ASSIGNMENTS FOR THE REDUCED FORM OF A REPEAT UNIT TETRASACCHARIDE DERIVED FROM KERATAN SULFATE BY KERATINASE DIGESTION AND PARTIAL ASSIGNMENTS FOR 2 FUCOSYLATED PENTASACCHARIDES

被引：13

作者：

HUCKERBY, TN ^{[1
]}

DICKENSON, JM ^{[1
]}

TAI, GH ^{[1
]}

LAUDER, RM ^{[1
]}

BROWN, GM ^{[1
]}

NIEDUSZYNSKI, IA ^{[1
]}

机构：

[1] UNIV LANCASTER,INST ENVIRONM & BIOL SCI,DIV BIOL SCI,LANCASTER LA1 4YQ,ENGLAND

来源：

MAGNETIC RESONANCE IN CHEMISTRY | 1993年 / 31卷 / 04期

关键词：

KERATAN SULFATE; C-13; NMR; C/H CORRELATION; OLIGOSACCHARIDES; STRUCTURAL ASSIGNMENT;

D O I：

10.1002/mrc.1260310416

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A full assignment of the 60-degrees-C C-13 NMR spectrum for the reduced form of a repeat unit beta-linked tetrasaccharide derived by keratanase digestion from keratan sulphate, GlcNAc(6SO4)beta1-3Gal(6SO4)beta1-4GlcNAc(6SO4)beta1-3Gal-ol, was achieved via two-dimensional C/H correlation and H-1 COSY experiments, and a complete first-order analysis of the 600 MHz proton spectrum. Partial C-13 assignments for two fucosylated keratan sulphate pentasccharides derived using hydrazinolysis and nitrous acid treatment are discussed.

引用

页码：394 / 398

页数：5

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