THE DAUGHTERLESS GENE-PRODUCT IN DROSOPHILA IS A NUCLEAR-PROTEIN THAT IS BROADLY EXPRESSED THROUGHOUT THE ORGANISM DURING DEVELOPMENT

被引:79
作者
CRONMILLER, C
CUMMINGS, CA
机构
[1] Department of Biology, University of Virginia, Charlottesville
关键词
DAUGHTERLESS; DROSOPHILA; HELIX-LOOP-HELIX;
D O I
10.1016/0925-4773(93)90005-I
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The daughterless (da) gene in Drosophila functions in the regulation of at least three significant developmental pathways: sex determination, neurogenesis and oogenesis. As a member of the helix-loop-helix (HLH) family of DNA binding proteins, the da gene product appears to act as a transcription factor. Based on the genetic and molecular characterization of da, it has been proposed that the da protein (Da) functions as a generic member of this family, serving throughout development as a necessary binding partner for an assortment of other HLH proteins. As a result of temporally and/or spatially restricted expression, these binding partners would provide some regulatory specificity to the functional transcription complex. In order to participate in this way in the regulation of multiple genes, Da must be expressed in numerous times and places during development. Using anti-Da antibodies, we validate two predictions of this scenario of Da function: (1) Da protein is not only nuclear localized, but also associated with chromosomes in vivo; and (2) Da protein is widely distributed, both spatially and temporally, throughout development. With regard to the essential role of maternal da+ in progeny sex determination, little, if any, Da protein is synthesized in the maternal germline. This suggests that the female-specific germline function of da+ is provided to the zygote as maternally synthesized RNA that becomes translated early in embryogenesis.
引用
收藏
页码:159 / 169
页数:11
相关论文
共 56 条
[41]  
Lindsley DL., 1992, GENOME DROSOPHILA ME
[42]  
MANGE AP, 1973, GENETICS, V73, P73
[43]   POLY(A) ELONGATION DURING XENOPUS OOCYTE MATURATION IS REQUIRED FOR TRANSLATIONAL RECRUITMENT AND IS MEDIATED BY A SHORT SEQUENCE ELEMENT [J].
MCGREW, LL ;
DWORKINRASTL, E ;
DWORKIN, MB ;
RICHTER, JD .
GENES & DEVELOPMENT, 1989, 3 (06) :803-815
[44]   A NEW DNA-BINDING AND DIMERIZATION MOTIF IN IMMUNOGLOBULIN ENHANCER BINDING, DAUGHTERLESS, MYOD, AND MYC PROTEINS [J].
MURRE, C ;
MCCAW, PS ;
BALTIMORE, D .
CELL, 1989, 56 (05) :777-783
[45]   INTERACTIONS BETWEEN HETEROLOGOUS HELIX-LOOP-HELIX PROTEINS GENERATE COMPLEXES THAT BIND SPECIFICALLY TO A COMMON DNA-SEQUENCE [J].
MURRE, C ;
MCCAW, PS ;
VAESSIN, H ;
CAUDY, M ;
JAN, LY ;
JAN, YN ;
CABRERA, CV ;
BUSKIN, JN ;
HAUSCHKA, SD ;
LASSAR, AB ;
WEINTRAUB, H ;
BALTIMORE, D .
CELL, 1989, 58 (03) :537-544
[46]   X-A RATIO, THE PRIMARY SEX-DETERMINING SIGNAL IN DROSOPHILA, IS TRANSDUCED BY HELIX-LOOP-HELIX PROTEINS [J].
PARKHURST, SM ;
BOPP, D ;
ISHHOROWICZ, D .
CELL, 1990, 63 (06) :1179-1191
[47]  
PARKHURST SM, 1993, DEVELOPMENT, V117, P737
[48]  
PIROTTA V, 1988, GENE DEV, V2, P1839
[49]   REGULATION OF ACHAETE-SCUTE GENE-EXPRESSION AND SENSORY ORGAN PATTERN-FORMATION IN THE DROSOPHILA WING [J].
SKEATH, JB ;
CARROLL, SB .
GENES & DEVELOPMENT, 1991, 5 (06) :984-995
[50]   REQUIREMENT FOR PHOSPHORYLATION AND LOCALIZATION OF THE BICAUDAL-D PROTEIN IN DROSOPHILA OOCYTE DIFFERENTIATION [J].
SUTER, B ;
STEWARD, R .
CELL, 1991, 67 (05) :917-926