EVALUATION OF A NEW METHOD FOR IDENTIFICATION OF CRYPTOCOCCUS-NEOFORMANS WHICH USES SEROLOGIC TESTS AIDED BY SELECTED BIOLOGICAL TESTS

被引:44
作者
KABASAWA, K
ITAGAKI, H
IKEDA, R
SHINODA, T
KAGAYA, K
FUKAZAWA, Y
机构
[1] YAMANASHI MED COLL,DEPT MICROBIOL,TAMAHO,YAMANASHI 40938,JAPAN
[2] MEIJI COLL PHARM,DEPT MICROBIOL,TANASHI,TOKYO 188,JAPAN
[3] IATRON LABS INC LTD,CHIYODA KU,TOKYO 101,JAPAN
关键词
D O I
10.1128/JCM.29.12.2873-2876.1991
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A new method for identifying Cryptococcus neoformans isolates and their serotypes by the slide agglutination test using five kinds of factor sera, with the aid of nitrate reduction, phenol oxidase, and growth at 37-degrees-C tests was evaluated by using 36 reference strains and 75 clinical isolates of C. neoformans. The results showed that the reference strains were identified exactly as they were labeled, and clinical isolates were identified as C. neoformans serotypes A, D, and AD. C. neoformans could be distinguished from other Cryptococcus species that cross-reacted with factor sera by their ability to grow at 37-degrees-C. These results indicate that the slide agglutination test combined the use of factor sera for isolates which grow at 37-degrees-C is a useful method for identification of C. neoformans and their serotypes and that the nitrate reduction test (negative in 100% of the isolates) and the phenol oxidase test (positive in approximately 95% of the isolates) can be used to confirm that the species is C. neoformans.
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页码:2873 / 2876
页数:4
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