HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF RUFLOXACIN AND ITS MAIN ACTIVE METABOLITE IN BIOLOGICAL-FLUIDS

被引：14

作者：

LOMBARDI, F

ARDEMAGNI, R

COLZANI, V

VISCONTI, M

机构：

[1] ISF, Laboratories for Biomedical Research, Milan, Via Leonardo da Vinci 1

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 576卷 / 01期

关键词：

D O I：

10.1016/0378-4347(92)80183-Q

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method is described for the determination of a fluoroquinolone, rufloxacin, and its N-desmethyl metabolite in plasma, urine and bile. Samples are chromatographed on a poly(styrene-divinylbenzene) column, the eluate being monitored with a fluorescence detector. The method was validated and a detection limit of 10 ng.ml for both rufloxacin and its metabolite in all the biological matrices considered was found. The method was successfully applied in pharmacokinetic studies.

引用

页码：129 / 134

页数：6

共 12 条

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