THE DETERMINATION OF THE MOLECULAR-WEIGHT DISTRIBUTION OF PECTINS BY CALIBRATED GPC .1. CALIBRATION BY LIGHT-SCATTERING AND MEMBRANE OSMOMETRY

被引：21

作者：

BERTH, G ^{[1
]}

DAUTZENBERG, H ^{[1
]}

LEXOW, D ^{[1
]}

ROTHER, G ^{[1
]}

机构：

[1] ACAD SCI GDR,INST POLYMER CHEM,TELTOW,GERMANY

来源：

CARBOHYDRATE POLYMERS | 1990年 / 12卷 / 01期

关键词：

D O I：

10.1016/0144-8617(90)90103-Y

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Commercial high methoxyl citrus pectin was fractionated on Sepharose 2 B/Sepharose 4 B. Fractions were used to calibrate GPC by light scattering and membrane osmometry. The angular dependence of light scattering of the fractions was interpreted in terms of a two-component system giving reliable Mw/Mn ratios for the major molecularly dispersed component which forms coils of considerable stiffness in solution. A minor particulate spherical component was identified which was rich in neutral sugars. Structural parameters of this component were determined by comparison of the experimental scattering behavior with master curves. β-Elimination of the fully esterified pectin was carried out to reveal the chemical nature of the particulate component. © 1989.

引用

页码：39 / 59

页数：21

共 41 条

[1] GEL-PERMEATION CHROMATOGRAPHY AND THE MARK-HOUWINK RELATION FOR PECTINS WITH DIFFERENT DEGREES OF ESTERIFICATION
ANGER, H
BERTH, G
[J]. CARBOHYDRATE POLYMERS, 1986, 6 (03) : 193 - 202
[2] Conformation of a low methoxyl citrus pectin in aqueous solution
Axelos, M. A. V.
Lefebvre, J.
Thibault, J. F.
[J]. FOOD HYDROCOLLOIDS, 1987, 1 (5-6) : 569 - 570
[3] BEACH P, 1986, ACS S SERIES, V310
[4] BERTACCINI G, 1982, MEDIATORS DRUGS GAST, V2, P1
[5] BERTH G, 1980, NAHRUNG, V24, P529, DOI 10.1002/food.19800240608
[6] BERTH G, 1977, NAHRUNG, V21, P939, DOI 10.1002/food.19770211012
[7] STUDIES ON THE HETEROGENEITY OF CITRUS PECTIN BY GEL-PERMEATION CHROMATOGRAPHY ON SEPHAROSE-2-B SEPHAROSE-4-B
BERTH, G
[J]. CARBOHYDRATE POLYMERS, 1988, 8 (02) : 105 - 117
[8] BERTH G, UNPUB
[9] STATIC AND DYNAMIC LIGHT-SCATTERING-STUDIES OF PECTIC POLYSACCHARIDES FROM THE MIDDLE LAMELLAE AND PRIMARY-CELL WALLS OF CIDER APPLES
CHAPMAN, HD
MORRIS, VJ
SELVENDRAN, RR
ONEILL, MA
[J]. CARBOHYDRATE RESEARCH, 1987, 165 (01) : 53 - 68
[10] INTERPRETATION OF LIGHT-SCATTERING FROM SUPERMOLECULAR STRUCTURES IN LIQUID-SYSTEMS BY MASTER CURVES
DAUTZENBERG, H
ROTHER, G
[J]. JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS, 1988, 26 (02) : 353 - 366

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