RANDOM SEQUENCING OF CDNA LIBRARIES REVEALS A VARIETY OF EXPRESSED GENES IN CULTURED-CELLS OF RICE (ORYZA-SATIVA L)

被引：93

作者：

UCHIMIYA, H

KIDOU, S

SHIMAZAKI, T

AOTSUKA, S

TAKAMATSU, S

NISHI, R

HASHIMOTO, H

MATSUBAYASHI, Y

KIDOU, N

UMEDA, M

KATO, A

机构：

[1] HOKKAIDO UNIV,DEPT BOT,SAPPORO,HOKKAIDO 060,JAPAN

[2] POLA CORP,PLANT BIOSCI LAB,TOTSUKA KU,YOKOHAMA 244,JAPAN

[3] FUKUI AGR EXPT STN,FUKUI 910,JAPAN

[4] NISSHINBO IND INC,TOKYO RES CTR,ADACHI KU,TOKYO 123,JAPAN

来源：

PLANT JOURNAL | 1992年 / 2卷 / 06期

关键词：

D O I：

10.1111/j.1365-313X.1992.01005.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Large scale sequencing of randomly selected cDNA clones was carried out to investigate the feasibility of this method for isolating plant genes. cDNA libraries were made using mRNA prepared from suspension-cultured cells of rice (Oryza sativa L.). Partial nucleotide sequences of 830 individual cDNA clones have been determined and compared with the GenBank database. Approximately 8% of the cDNA clones could be identified as particular genes. This method provides the opportunity to isolate large numbers of plant genes.

引用

页码：1005 / 1009

页数：5

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