PREPARATION OF D-SORBOSE FROM D-TAGATOSE BY IMMOBILIZED D-TAGATOSE 3-EPIMERASE

被引：53

作者：

ITOH, H ^{[1
]}

SATO, T ^{[1
]}

TAKEUCHI, T ^{[1
]}

KHAN, AR ^{[1
]}

IZUMORI, K ^{[1
]}

机构：

[1] KAGAWA UNIV,FAC AGR,DEPT BIORESOURCE SCI,MIKI,KAGAWA 76107,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1995年 / 79卷 / 02期

关键词：

D-SORBOSE PREPARATION; IMMOBILIZATION; D-TAGATOSE; 3-EPIMERASE; PSEUDOMONAS SP ST-24;

D O I：

10.1016/0922-338X(95)94091-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

D-Tagatose 3-epimerase (D-TE) from Pseudomonas sp. ST-24 was immobilized on various types of Chitopearl beads. The highest activity was found in D-TE immobilized on Chitopearl beads of BCW 2503, the yield being about 80% of free enzyme applied. Maximum activity of the immobilized enzyme was obtained at pH 7-9 and around 60 degrees C. The enzyme was stable in a pH range of 7-10, and below 60 degrees C. In a high concentration (30%) of substrate, the reaction progressed without substrate inhibition. Two grams of D-sorbose crystals could be obtained from 3 g D-tagatose. Furthermore, in a batch reaction repeated five times, about 70% of D-tagatose was converted to D-sorbose each time.

引用

页码：184 / 185

页数：2

共 5 条

[1] PREPARATION OF L-FRUCTOSE AND D-SORBOSE BY THE BACTERIAL OXIDATION OF L-MANNITOL AND L-GLUCITOL, RESPECTIVELY [J].

DHAWALE, MR ;

SZAREK, WA ;

HAY, GW ;

KROPINSKI, AMB .

CARBOHYDRATE RESEARCH, 1986, 155 :262-265

[2] PURIFICATION AND CHARACTERIZATION OF D-TAGATOSE 3-EPIMERASE FROM PSEUDOMONAS SP, ST-24 [J].

ITOH, H ;

OKAYA, H ;

KHAN, AR ;

TAJIMA, S ;

HAYAKAWA, S ;

IZUMORI, K .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1994, 58 (12) :2168-2171

[3] A NEW ENZYME, D-KETOHEXOSE 3-EPIMERASE, FROM PSEUDOMONAS SP ST-24 [J].

IZUMORI, K ;

KHAN, AR ;

OKAYA, H ;

TSUMURA, T .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1993, 57 (06) :1037-1039

[4]

KHAN AR, 1992, J FERMENT BIOENG, V74, P144

[5]

MAUNIRUZZAMAN S, 1994, J FERMENT BIOENG, V78, P145

← 1 →