NERVE GROWTH-FACTOR RECEPTORS ARE PREAGGREGATED AND IMMOBILE ON RESPONSIVE CELLS

被引:16
作者
VENKATAKRISHNAN, G [1 ]
MCKINNON, CA [1 ]
PILAPIL, CG [1 ]
WOLF, DE [1 ]
ROSS, AH [1 ]
机构
[1] WORCESTER FDN EXPTL BIOL INC, 222 MAPLE AVE, SHREWSBURY, MA 01545 USA
关键词
D O I
10.1021/bi00225a002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It has been hypothesized that signal transduction occurs by ligand-induced receptor clustering and immobilization. For many peptide receptors, cross-linking by anti-receptor antibodies is sufficient for receptor activation. This is not, however, the case for nerve growth factor receptor (NGFR). Using fluorescence microscopy and fluorescence recovery after photobleaching (FRAP), we have analyzed the distribution and diffusibility of NGFR on a series of cell lines. We have found the following: (1) Cells expressing high-affinity responsive NGFR's display clustered NGFR's even in the absence of ligand. In contrast, NGFR's in nonresponsive cell lines are diffusely distributed. (2) Receptors on responsive cell lines are largely nondiffusing while most receptors on nonresponsive cell lines are relatively free to diffuse. (3) NGF does not greatly alter the distribution or diffusion properties of the NGFR on either nonresponsive or responsive cell lines. Thus, NGFR is preclustered and immobile on responsive cells, which suggests that immobilization of NGFR prior to ligand binding is required for signal transduction.
引用
收藏
页码:2748 / 2753
页数:6
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