SELECTIVE PROTEOLYSIS OF THE WHEAT EM POLYPEPTIDE - IDENTIFICATION OF AN ENDOPEPTIDASE ACTIVITY IN GERMINATING WHEAT EMBRYOS

被引：11

作者：

TAYLOR, RM ^{[1
]}

CUMING, AC ^{[1
]}

机构：

[1] UNIV LEEDS,DEPT GENET,LEEDS LS2 9JT,W YORKSHIRE,ENGLAND

来源：

FEBS LETTERS | 1993年 / 331卷 / 1-2期

关键词：

EM POLYPEPTIDE; CYSTEINE PROTEINASE; WHEAT EMBRYO; GERMINATION;

D O I：

10.1016/0014-5793(93)80299-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The 'Em' polypeptide is the most abundant cytosolic polypeptide in mature wheat embryos. It is selectively and completely degraded within the first 24 h of germination. Extracts from germinated embryos contain endopeptidase activities which degrade the Em polypeptide. These are separable into a major and minor component by ion-exchange chromatography and the use of inhibitors shows the major component to be a cysteine proteinase. This activity shows a strong preference for the Em polypeptide as a substrate, being inactive against polypeptides which are not developmentally regulated and showing only low activity towards developmentally related, but otherwise nonhomologous 'dehydrin' polypeptides.

引用

页码：71 / 75

页数：5

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