HIGH SOMATIC MUTATION FREQUENCIES IN A LACZ TRANSGENE INTEGRATED ON THE MOUSE X-CHROMOSOME

被引:51
作者
GOSSEN, JA
DELEEUW, WJF
VERWEST, A
LOHMAN, PHM
VIJG, J
机构
[1] LEIDEN UNIV,TNO,INST AGEING & VASCULAR RES,2333 AL LEIDEN,NETHERLANDS
[2] LEIDEN UNIV,DEPT RADIAT GENET & CHEM MUTAGENESIS,2333 AL LEIDEN,NETHERLANDS
来源
MUTATION RESEARCH | 1991年 / 250卷 / 1-2期
关键词
TRANSGENIC MICE; SPONTANEOUS MUTATIONS; LACZ SHUTTLE VECTOR; MUTATIONAL HOT SPOT; MOUSE X-CHROMOSOME;
D O I
10.1016/0027-5107(91)90198-W
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
To study spontaneous and induced mutagenesis in vivo we recently constructed a series of transgenic mice harboring different numbers of bacteriophage lambda shuttle vectors, provided with a LacZ mutational target gene, integrated in their genome. The transgenic mice enabled analysis of spontaneous and induced mutation frequencies in postmitotic tissues like liver and brain. The obtained data indicated spontaneous mutation frequencies in the order of 10(-5)-10(-6). Here we report a 25-100 times higher spontaneous mutation frequency in liver and brain DNA of mice from strain 35.5, with the lambda-gt10LacZ concatemer integrated on the X-chromosome. These results indicate the presence of a mutational 'hot spot' in the mammalian somatic genome in vivo.
引用
收藏
页码:423 / 429
页数:7
相关论文
共 24 条
[11]   CHARACTERIZATION OF A HIGHLY UNSTABLE MOUSE MINISATELLITE LOCUS - EVIDENCE FOR SOMATIC MUTATION DURING EARLY DEVELOPMENT [J].
KELLY, R ;
BULFIELD, G ;
COLLICK, A ;
GIBBS, M ;
JEFFREYS, AJ .
GENOMICS, 1989, 5 (04) :844-856
[12]   FIDELITY OF DNA-POLYMERASES IN DNA AMPLIFICATION [J].
KEOHAVONG, P ;
THILLY, WG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (23) :9253-9257
[13]  
LOEB LA, 1989, CANCER RES, V49, P5489
[14]   DNA METHODS FOR DETECTING AND ANALYZING MUTATIONS INVIVO [J].
LOHMAN, PHM ;
VIJG, J ;
UITTERLINDEN, AG ;
SLAGBOOM, P ;
GOSSEN, JA ;
BERENDS, F .
MUTATION RESEARCH, 1987, 181 (02) :227-234
[15]   MEASUREMENT OF INVIVO MUTATIONS IN HUMAN-LYMPHOCYTES [J].
MORLEY, AA ;
TRAINOR, KJ ;
SESHADRI, R ;
RYALL, RG .
NATURE, 1983, 302 (5904) :155-156
[16]   GERM-LINE TRANSFORMATION OF MICE [J].
PALMITER, RD ;
BRINSTER, RL .
ANNUAL REVIEW OF GENETICS, 1986, 20 :465-499
[17]   A GRADIENT OF SEX LINKAGE IN THE PSEUDOAUTOSOMAL REGION OF THE HUMAN SEX-CHROMOSOMES [J].
ROUYER, F ;
SIMMLER, MC ;
JOHNSSON, C ;
VERGNAUD, G ;
COOKE, HJ ;
WEISSENBACH, J .
NATURE, 1986, 319 (6051) :291-295
[18]  
SCHMIDT M, 1990, P NATL ACAD SCI USA, V887, P3685
[19]   HPRT - GENE STRUCTURE, EXPRESSION, AND MUTATION [J].
STOUT, JT ;
CASKEY, CT .
ANNUAL REVIEW OF GENETICS, 1985, 19 :127-148
[20]   THE MOLECULAR NATURE OF MUTATIONS IN CULTURED MAMMALIAN-CELLS - A REVIEW [J].
THACKER, J .
MUTATION RESEARCH, 1985, 150 (1-2) :431-442