ANALYSIS OF LEGUME OLIGOSACCHARIDES BY HIGH-RESOLUTION GAS-CHROMATOGRAPHY

被引：9

作者：

KAROUTIS, AI

TYLER, RT

SLATER, GP

机构：

[1] UNIV SASKATCHEWAN,DEPT APPL MICROBIOL & FOOD SCI,FOOD PROC RES LAB,SASKATOON S7N 0W0,SASKATCHEWAN,CANADA

[2] NATL RES COUNCIL CANADA,INST PLANT BIOTECHNOL,SASKATOON S7N 0W9,SASKATCHEWAN,CANADA

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 623卷 / 01期

关键词：

D O I：

10.1016/0021-9673(92)85317-M

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The suitability of high-resolution gas chromatography (HGRC) for the analysis of the raffinose family oligosaccharides (raffinose, stachyose, verbascose) was investigated. Aqueous methanol (80%) extracts of pea flour were dried and derivatized with either trimethylimidazole or N-methyl-bis(trifluoroacetamide). Separation of the sugar derivatives was achieved utilizing a 10-m DBS-60W capillary column. The effects of carrier gas (He) flow-rate and split ratio on resolution and reproducibility were studied. HRGC analysis was characterized by excellent resolution and satisfactory reproducibility, and proved to be a rapid, sensitive method for quantitation of oligosaccharides in pea flours.

引用

页码：186 / 190

页数：5

共 8 条

[1] TRIFLUOROACETYLATION OF CARBOHYDRATES FOR GLC, USING N-METHYLBIS(TRIFLUOROACETAMIDE) [J].