COMPARISON OF METHODS TO DETERMINE ALIPHATIC CHAIN-LENGTH IN BIOMEMBRANES FROM SELECTIVELY OR PERDEUTERATED SYSTEMS - A H-2-NMR STUDY

被引：5

作者：

DOULIEZ, JP ^{[1
]}

DUFOURC, EJ ^{[1
]}

机构：

[1] CNRS,CTR RECH PAUL PASCAL,F-33600 PESSAC,FRANCE

来源：

JOURNAL DE CHIMIE PHYSIQUE ET DE PHYSICO-CHIMIE BIOLOGIQUE | 1995年 / 92卷 / 10期

关键词：

BIOMEMBRANES; PERDEUTERATION; H-2-NMR; BOND ORDER PARAMETERS;

D O I：

10.1051/jcp/1995921721

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Comparison of the different methods to determine aliphatic chain length in biomembranes from the C-D quadrupolar splittings obtained with solid state H-2-NMR is reported. It is shown that perdeuteration of the saturated lipid acyl chains is the alternative to selectively labelled chains without loss of accuracy. Accuracy in chain length determination may be then estimated to be of the order of 0.2 Angstrom.

引用

页码：1721 / 1726

页数：6

共 16 条

[11] DEUTERIUM NUCLEAR MAGNETIC-RESONANCE INVESTIGATION OF EFFECTS OF PROTEINS AND POLYPEPTIDES ON HYDROCARBON CHAIN ORDER IN MODEL MEMBRANE SYSTEMS [J].