Detection of Leishmania in the blood of early kala-azar patients with the aid of the polymerase chain reaction

被引:55
作者
Adhya, S [1 ]
Chatterjee, M [1 ]
Hassan, MQ [1 ]
Mukherjee, S [1 ]
Sen, S [1 ]
机构
[1] SEN MED RES CTR,PATNA 800001,BIHAR,INDIA
关键词
visceral leishmaniasis; Leishmania donovani; diagnosis; ELISA; PCR; microscopy; India;
D O I
10.1016/0035-9203(95)90416-6
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Samples from 39 patients with symptoms suggestive of early visceral leishmaniasis were independently assayed by microscopy of tissue smears, enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) of blood deoxyribonucleic acid. Of these patients, 19 were confirmed as positive or negative by all 3 tests; 11 patients (28%) negative by smear were positive by ELISA and PCR; and 7 (18%) were positive by PCR alone. These results demonstrate the high sensitivity of the nbn-invasive PCR and, to a smaller extent, ELISA, in the early diagnosis of visceral leishmaniasis.
引用
收藏
页码:622 / 624
页数:3
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