IMMUNOLOGICAL STUDY OF ANTI-M2 IN ANTIMITOCHONDRIAL ANTIBODY-NEGATIVE PRIMARY BILIARY-CIRRHOSIS

被引：39

作者：

KITAMI, N

KOMADA, T

ISHII, H

SHIMIZU, H

ADACHI, H

YAMAGUCHI, Y

KITAMURA, T

OIDE, H

MIYAZAKI, A

ISHIKAWA, M

ENDO, K

WATANABE, S

YOKOI, Y

SATO, N

机构：

[1] Department of Gastroenterology, Juntendo University, School of Medicine, Tokyo

来源：

INTERNAL MEDICINE | 1995年 / 34卷 / 06期

关键词：

M2; PROTEIN; PYRUVATE DEHYDROGENASE COMPLEX; PROTEIN X; ANTINUCLEAR ANTIBODY; SMOOTH MUSCLE ANTIBODY; ANTICENTROMERE ANTIBODY;

D O I：

10.2169/internalmedicine.34.496

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We investigated the specificity of various autoantibodies in antimitochondrial antibody (AMA)-negative patients with primary biliary cirrhosis (PBC), We examined sera from 144 patients with PBC, 17 of whom were AMA negative by indirect immunofluorescence. The AMA-negative group showed a significantly higher positivity for smooth muscle antibody, but not for antinuclear antibody, as compared with the AMA-positive group, IgG class anti-PDH by enzyme-linked immunosorbent assay (ELISA) mere detected in 13 % of the AMA-negative group, However, all PBC patients showed positive IgG, IgA, and/or IgM class anti-M2 to the four M2 proteins by immunoblotting. These results suggest that the detection of IgG and IgM class anti-PDH and that of antibodies to the four M2 proteins increases the positivity of this ELISA method, and that detection of IgG, IgA, and IgM class anti-M2 to the four M2 proteins by immunoblotting is useful in diagnosing AMA-negative patients with PBC.

引用

页码：496 / 501

页数：6

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