SIMPLE FED-BATCH TECHNIQUE FOR HIGH CELL-DENSITY CULTIVATION OF ESCHERICHIA-COLI

被引：376

作者：

KORZ, DJ ^{[1
]}

RINAS, U ^{[1
]}

HELLMUTH, K ^{[1
]}

SANDERS, EA ^{[1
]}

DECKWER, WD ^{[1
]}

机构：

[1] GBF,NATL RES CTR BIOTECHNOL,DIV BIOCHEM ENGN,D-38124 BRAUNSCHWEIG,GERMANY

来源：

JOURNAL OF BIOTECHNOLOGY | 1995年 / 39卷 / 01期

关键词：

ESCHERICHIA COLI; FED-BATCH; CULTIVATION; HIGH CELL DENSITY;

D O I：

10.1016/0168-1656(94)00143-Z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A simple fed-batch process for high cell density cultivation of Escherichia coli TG1 was developed. A pre-determined feeding strategy was chosen to maintain carbon-limited growth using a defined medium. Feeding was carried out to increase the cell mass concentration exponentially in the bioreactor controlling biomass accumulation at growth rates which do not cause the formation of acetic acid (mu < mu(crit)). Cell concentrations of 128 and 148 g per 1 dry cell weight (g l(-1) DCW) were obtained using glucose or glycerol as carbon source, respectively.

引用

页码：59 / 65

页数：7

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