CLONING A NOVEL HUMAN BRAIN INWARD RECTIFIER POTASSIUM CHANNEL AND ITS FUNCTIONAL EXPRESSION IN XENOPUS-OOCYTES

被引：39

作者：

TANG, WM

YANG, XC

机构：

[1] AMER CYANAMID CO, LEDERLE LABS, DEPT CNS RES, DIV MED RES, PEARL RIVER, NY 10965 USA

[2] COLUMBIA UNIV, COLL PHYS & SURG, DEPT PHARMACOL, NEW YORK, NY 10032 USA

来源：

FEBS LETTERS | 1994年 / 348卷 / 03期

关键词：

INWARD RECTIFIER K+ CHANNEL; HUMAN FRONTAL CORTEX; CDNA; TISSUE SPECIFICITY; N-SHAPE IV CURVE; XENOPUS OOCYTE;

D O I：

10.1016/0014-5793(94)00612-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned a novel inward rectifier K+ channel (hIRK2) from a human frontal cortex cDNA library. The amino acid sequence of hIRK2 shares 60% and 40% identity with the mouse IRK1 and the rat ROMK1 channels, respectively; Xenopus oocytes injected with hIRK2 cRNA showed an inwardly rectifying K+ current that had a prominent 'N-shape' I-V curve and was blocked by extracellular Ba2+. The hIRK2 channel has two unique features: (a) an 18 amino acid insertion between the first transmembrane region and the pore, and (b) restricted mRNA distribution found only in human brain and heart.

引用

页码：239 / 243

页数：5