LACTATE-DEHYDROGENASE AS SAFE END-POINT COOKING INDICATOR IN POULTRY BREAST ROLLS - DEVELOPMENT OF MONOCLONAL-ANTIBODIES AND APPLICATION TO SANDWICH ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA)

被引:19
作者
ABOUZIED, MM [1 ]
WANG, CH [1 ]
PESTKA, JJ [1 ]
SMITH, DM [1 ]
机构
[1] MICHIGAN STATE UNIV, DEPT FOOD SCI & HUMAN NUTR, E LANSING, MI 48824 USA
关键词
D O I
10.4315/0362-028X-56.2.120
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A sandwich enzyme-linked immunosorbent assay (ELISA) was developed to detect lactate dehydrogenase (LDH) as a marker protein for verifying endpoint cooking of uncured poultry products. Monoclonal antibodies were prepared against chicken muscle LDH and used with rabbit polyclonal antibodies developed against turkey or chicken muscle LDH for capture and detection in the assay, respectively. Minimum assay detection limits for turkey and chicken muscle LDH were 1 ng/ml. Turkey and chicken muscle LDH, but not LDH from other species cross reacted in the ELISA. The ELISA was further verified using extracts of turkey breast rolls processed to internal temperatures between 68.3 and 72.1-degrees-C. The LDH content of extracts diluted 3- to 6-fold was below 15 ng/ml for turkey rolls processed to 70.9 and 72.1-degrees-C. At a 6-fold dilution, LDH content of extracts from rolls processed to 69.7-degrees-C was approximately 10 times greater than those processed to 70.9-degrees-C. A survey of market precooked poultry products indicated assay validity with precooked turkey roast, but not turkey hams with maximum internal temperature requirements of 68.3-degrees-C. Results suggested the sandwich ELISA should be applicable for determining whether turkey breast rolls are processed to the required U.S. Department of Agriculture endpoint temperature of 71.1-degrees-C.
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页码:120 / +
页数:1
相关论文
共 25 条
[1]   MONOCLONAL ANTIBODY-BASED ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR C-19-DELTA-16-STEROIDS IN SERA OF BOAR PIGS [J].
ABOUZIED, MM ;
ASGHAR, A ;
PEARSON, AM ;
GRAY, JI ;
MILLER, ER ;
PESTKA, JJ .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1990, 38 (01) :331-335
[2]   THE IMMUNOCHEMISTRY OF SANDWICH ELISAS .1. THE BINDING CHARACTERISTICS OF IMMUNOGLOBULINS TO MONOCLONAL AND POLYCLONAL CAPTURE ANTIBODIES ADSORBED ON PLASTIC AND THEIR DETECTION BY SYMMETRICAL AND ASYMMETRICAL ANTIBODY-ENZYME CONJUGATES [J].
BUTLER, JE ;
SPRADLING, JE ;
SUTER, M ;
DIERKS, SE ;
HEYERMANN, H ;
PETERMAN, JH .
MOLECULAR IMMUNOLOGY, 1986, 23 (09) :971-982
[3]   QUANTITATIVE-DETERMINATION OF LOW-SALT SOLUBLE-PROTEIN PATTERNS OF BOVINE MUSCLES COOKED AT DIFFERENT TEMPERATURES, BY SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL-ELECTROPHORESIS [J].
CALDIRONI, HA ;
BAZAN, NG .
JOURNAL OF FOOD SCIENCE, 1980, 45 (04) :901-904
[4]   LACTATE-DEHYDROGENASE ACTIVITY IN BOVINE MUSCLE AS A POTENTIAL HEATING END-POINT INDICATOR [J].
COLLINS, SS ;
KEETON, JT ;
SMITH, SB .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1991, 39 (07) :1291-1293
[5]   LACTATE-DEHYDROGENASE ENZYME-ACTIVITY IN RAW, CURED, AND HEATED PORCINE MUSCLE [J].
COLLINS, SS ;
KEETON, JT ;
SMITH, SB .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1991, 39 (07) :1294-1297
[6]   SIZE EXCLUSION HPLC OF HEATED WATER-SOLUBLE BOVINE AND PORCINE MUSCLE PROTEINS [J].
DAVIS, CE ;
ANDERSON, JB .
JOURNAL OF FOOD SCIENCE, 1984, 49 (02) :598-602
[7]   PYRUVATE-KINASE ACTIVITY AS AN INDICATOR OF TEMPERATURE ATTAINED DURING COOKING OF CURED PORK [J].
DAVIS, CE ;
SEARCY, GK ;
BLANKENSHIP, LC ;
TOWNSEND, WE .
JOURNAL OF FOOD PROTECTION, 1988, 51 (10) :773-&
[8]  
GALFRE G, 1981, METHOD ENZYMOL, V73, P1
[9]   ANTIBODY-PRODUCTION BY HYBRIDOMAS [J].
GODING, JW .
JOURNAL OF IMMUNOLOGICAL METHODS, 1980, 39 :285-308
[10]  
HAMM REINER, 1960, FOOD RES, V25, P587