THE FUNCTION OF ATP-ADP TRANSLOCATOR IN THE REGULATION OF MITOCHONDRIAL RESPIRATION DURING DEVELOPMENT OF HEART ISCHEMIC-INJURY

被引：13

作者：

BORUTAITE, V ^{[1
]}

MILDAZIENE, V ^{[1
]}

KATILIUTE, Z ^{[1
]}

KHOLODENKO, B ^{[1
]}

TOLEIKIS, A ^{[1
]}

机构：

[1] MOSCOW MV LOMONOSOV STATE UNIV, AN BELOZERSKY INST PHYS CHEM BIOL, MOSCOW, RUSSIA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1142卷 / 1-2期

关键词：

MITOCHONDRION; ISCHEMIA; RESPIRATION; CONTROL COEFFICIENT; HEART;

D O I：

10.1016/0005-2728(93)90099-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Inhibitor titration studies were carried out in order to quantify the amount of control exerted by ATP/ADP translocator on the rate of succinate, palmitoylcamitine + malate and pyruvate + malate oxidation in ischemia-damaged heart mitochondria. It was shown that after 30 min of total ischemia in vitro the maximal value of the control coefficient of the translocator was as high as in the control: 0.5-0.72 (succinate), 0.8-0.87 (palmitoylcarnitine + malate), 0.83-0.95 (pyruvate + malate). However, the translocator-controlled range of respiratory rates of ischemic mitochondria was narrower than that of normal mitochondria. The control coefficient of the translocator close to State 3 and State 4 was equal to 0-0.15. After 45 min ischemia the maximal value of the translocator control coefficient decreased by 25-30% in comparison with normal mitochondria with all substrates investigated. This value was preserved within a wide range of mitochondrial respiratory rates including the maximal rate in State 3. It was found that the amount of ATP/ADP translocator in mitochondria decreased by 20% after only 45 min ischemia. Our data show that the ATP/ADP translocator is one of the most important steps in regulation of oxidative phosphorylation in isolated mitochondria during development of heart ischemic injury.

引用

页码：175 / 180

页数：6

共 31 条

[11] JENNINGS RB, 1981, AM J PATHOL, V102, P241
[12] Kacser H, 1973, Symp Soc Exp Biol, V27, P65
[13] THE ROLE OF ADENINE-NUCLEOTIDE TRANSLOCATORS IN REGULATION OF OXIDATIVE-PHOSPHORYLATION IN HEART-MITOCHONDRIA
KHOLODENKO, B
ZILINSKIENE, V
BORUTAITE, V
IVANOVIENE, L
TOLEIKIS, A
PRASKEVICIUS, A
[J]. FEBS LETTERS, 1987, 223 (02) : 247 - 250
[14] KHOLODENKO BN, 1991, BIOKHIMIJIA, V50, P1420
[15] KLINGENBERG M, 1982, METABOLIC COMPARTMEN, P101
[16] ADENINE-NUCLEOTIDE TRANSPORT DURING CARDIAC ISCHEMIA
LANOUE, KF
WATTS, JA
KOCH, CD
[J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1981, 241 (05): : H663 - H671
[17] LAWSON JWR, 1979, J BIOL CHEM, V254, P6528
[18] MINATOGUCHI H, 1984, JPN HEART J, V25, P609
[19] MITTNACHT S, 1979, J BIOL CHEM, V254, P9871
[20] DEVELOPMENT OF ISCHEMIA-INDUCED DAMAGE IN DEFINED MITOCHONDRIAL SUBPOPULATIONS
PIPER, HM
SEZER, O
SCHLEYER, M
SCHWARTZ, P
HUTTER, JF
SPIECKERMANN, PG
[J]. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 1985, 17 (09) : 885 - 896

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