PURIFICATION OF FKBP-70, A NOVEL IMMUNOPHILIN FROM SACCHAROMYCES-CEREVISIAE, AND CLONING OF ITS STRUCTURAL GENE, FPR3

被引：25

作者：

MANNINGKRIEG, UC ^{[1
]}

HENRIQUEZ, R ^{[1
]}

CAMMAS, F ^{[1
]}

GRAFF, P ^{[1
]}

GAVERIAUX, S ^{[1
]}

MOVVA, NR ^{[1
]}

机构：

[1] SANDOZ PHARMA LTD,PRECLIN RES,CH-4002 BASEL,SWITZERLAND

来源：

FEBS LETTERS | 1994年 / 352卷 / 01期

关键词：

IMMUNOSUPPRESSION; FK506; PPIASE; ASCOMYCIN; YEAST; CSA;

D O I：

10.1016/0014-5793(94)00927-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel protein, belonging to the yeast family of FKBPs (FK-binding proteins), FKBP-70, was isolated from Saccharomyces cerevisiae by its interaction with the immunosuppressive agent FK-520. Its structural gene, FPR3, was cloned and the protein expressed and purified from Escherichia coli. This third member of the FKBP family in yeast is homologous to the other FKBPs at its carboxy terminus, showing conserved ligand binding and proline isomerase regions. It is, however, a longer acidic protein with several potential nuclear targeting sequences and a region of homology to nucleolins. Yeast strains deleted for FPR3, as well as a triple deletion mutant of this family of genes, FPR1, FPR2 and FPR3, are viable under normal conditions of growth, indicating that the FPR genes are not essential for life.

引用

页码：98 / 103

页数：6

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