ENZYMATIC PRODUCTION OF TRYPTOPHAN USING A LIPOIC ACID AND THIAMINE DOUBLE AUXOTROPH OF ENTEROBACTER-AEROGENES HAVING BOTH PYRUVIC-ACID PRODUCTIVITY AND HIGH TRYPTOPHANASE ACTIVITY

被引：5

作者：

OITA, S ^{[1
]}

YOKOTA, A ^{[1
]}

TAKAO, S ^{[1
]}

机构：

[1] HOKKAIDO UNIV,FAC AGR,DEPT AGR CHEM,SAPPORO,HOKKAIDO 060,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1990年 / 69卷 / 04期

关键词：

D O I：

10.1016/0922-338X(90)90223-J

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Washed cells of a newly-developed double auxotroph for lipoic acid and thiamine, Enterobacter aerogenes LT-94, produced about 30 g/l of pyruvic acid from 5% glucose, and subsequently converted the acid to tryptophan in a yield of 16.7 g/l by the reverse reaction of tryptophanase, which had been induced under derepressed conditions. These yields were higher than those of the parent strain, L-12, showing only lipoic acid auxotrophy. © 1990.

引用

页码：256 / 258

页数：3

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