ELECTROCHEMICAL DETERMINATION OF THE KINETIC-PARAMETERS OF MUSHROOM TYROSINASE

被引：21

作者：

RIVAS, G

SOLIS, VM

机构：

[1] Instituto de Investigaciones en Fisicoquimica de Cordoba (INFIQC), Departamento de Fisico Quimica, Facultad de Ciencias Quimicas, 5016 Cordoba, Sucursal 16

来源：

BIOELECTROCHEMISTRY AND BIOENERGETICS | 1992年 / 29卷 / 01期

关键词：

D O I：

10.1016/0302-4598(92)80050-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for determining K(m) of mushroom tyrosinase as well as the maximum velocity using L-dopa and L-tyrosine as substrates is described. The technique is based on measurements of the oxidation current of L-dopa which is both the substrate for the catecholase activity and the product of the enzymatic oxidation Of L-tyrosine. The oxygen concentration in the solutions is kept constant al the saturation level with air. A vitreous carbon electrode activated in 1 M KOH is employed. The values of the catalytic parameters obtained here with the voltammetric technique are comparable with those obtained by other authors under similar operative conditions using the spectroscopic method.

引用

页码：19 / 28

页数：10

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