DETERMINATION OF ARGINASE ACTIVITY IN MACROPHAGES - A MICROMETHOD

被引：460

作者：

CORRALIZA, IM

CAMPO, ML

SOLER, G

MODOLELL, M

机构：

[1] UNIV EXTREMADURA,FAC VET,DEPT BIOQUIM & BIOL MOLEC & GENET,E-10071 CACERES,SPAIN

[2] MAX PLANCK INST IMMUNBIOL,D-79108 FREIBURG,GERMANY

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1994年 / 174卷 / 1-2期

关键词：

MACROPHAGE; ARGINASE;

D O I：

10.1016/0022-1759(94)90027-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We propose a modification of Schimke's method for urea determination as a valuable micromethod for measuring arginase in activated macrophages. The method exhibits the following advantages: (a) it uses small amounts of samples (approximately 25000 macrophages per assay); (b) it does not interfere with other related metabolites that are also present in the activated macrophage such as citrulline or arginine; (c) saturating concentrations of the substrate arginine can be used; and (d) it is much more sensitive than Schimke's method and can detect small amounts of urea, in the order of 0.02 mu mol.

引用

页码：231 / 235

页数：5

共 16 条

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