SIMULTANEOUS DETERMINATION OF ALLANTOIN AND OXYPURINES IN BIOLOGICAL-FLUIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：245

作者：

BALCELLS, J ^{[1
]}

GUADA, JA ^{[1
]}

PEIRO, JM ^{[1
]}

PARKER, DS ^{[1
]}

机构：

[1] UNIV NEWCASTLE UPON TYNE,FAC AGR & BIOL SCI,DEPT BIOL & NUTR SCI,NEWCASTLE TYNE NE1 7RU,TYNE & WEAR,ENGLAND

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 575卷 / 01期

关键词：

D O I：

10.1016/0378-4347(92)80517-T

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method is described for the separation and quantification of allantoin and oxypurines in plasma and urine samples. Urine was analyzed directly and plasma after acid deproteinisation with perchloric acid. Separation and quantification of purine derivatives was achieved using two Spherisorb ODS-5 column (250 mm x 4.6 mm I.D.) connected in series together with a NH4H2PO4-NH4H2PO4-acetonitrile (80:20) gradient and monitoring the effluent at 205 nm. The average recoveries of standard compounds added to urine and plasma samples were 96 and 97%, respectively, using allopurinol as internal standard. The within-day variability was less than 7% and the day-to-day coefficient of variation less than 11% indicating a good precision of the method.

引用

页码：153 / 157

页数：5