FIXATION OF A HIGHLY REACTIVE FORM OF ALPHA-CHYMOTRYPSIN BY MICELLAR MATRIX

被引：24

作者：

LEVASHOV, AV ^{[1
]}

KLYACHKO, NL ^{[1
]}

BOGDANOVA, NG ^{[1
]}

MARTINEK, K ^{[1
]}

机构：

[1] INST ORGAN CHEM & BIOCHEM, PRAGUE, CZECHOSLOVAKIA

来源：

FEBS LETTERS | 1990年 / 268卷 / 01期

关键词：

Conformation; Solvated reversed micelle; Substrate specificity; α-Chymotrypsin;

D O I：

10.1016/0014-5793(90)81017-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using reversed micelles of surfactants solvated by water-organic cosolvent mixtures as a matrix for enzyme entrapping, it is possible to fix the highly reactive α-chymotrypsin form. The reactivity of α-chymotrypsin towards nonspecific substrates increases to the extent comparable with that observed in reactions involving specific substrates. © 1990.

引用

页码：238 / 240

页数：3

共 14 条

[1]

BELONOGOVA O V, 1983, Biokhimiya, V48, P379

[2] SPECIFICITY OF ALPHA-CHYMOTRYSPIN [J].

BEREZIN, IV ;

MARTINEK, K .

FEBS LETTERS, 1970, 8 (05) :261-&

[3]

BEREZIN IV, 1977, PHYSICAL CHEM ENZYMA

[4]

BEREZIN IV, 1974, STRUCTURE FUNCTIONS, P5

[5]

BOGDANOVA NG, 1989, BIOORG KHIM+, V15, P634

[6] FORMATION OF WATER-OIL-MICROEMULSIONS [J].

EICKE, HF ;

REHAK, J .

HELVETICA CHIMICA ACTA, 1976, 59 (08) :2883-2891

[7]

FERSHT A, STRUCTURE MECHANISM

[8]

KLYACHKO N L, 1989, Biokhimiya, V54, P1224

[9]

KLYACHKO NL, 1990, BIOORG KHIM+, V16, P581

[10] SOLUBILIZATION OF ENZYMES AND NUCLEIC-ACIDS IN HYDROCARBON MICELLAR SOLUTIONS [J].

LUISI, PL ;

MAGID, LJ .

CRC CRITICAL REVIEWS IN BIOCHEMISTRY, 1986, 20 (04) :409-474

← 1 2 →