FIXATION OF A HIGHLY REACTIVE FORM OF ALPHA-CHYMOTRYPSIN BY MICELLAR MATRIX

被引：24

作者：

LEVASHOV, AV ^{[1
]}

KLYACHKO, NL ^{[1
]}

BOGDANOVA, NG ^{[1
]}

MARTINEK, K ^{[1
]}

机构：

[1] INST ORGAN CHEM & BIOCHEM, PRAGUE, CZECHOSLOVAKIA

来源：

FEBS LETTERS | 1990年 / 268卷 / 01期

关键词：

Conformation; Solvated reversed micelle; Substrate specificity; α-Chymotrypsin;

D O I：

10.1016/0014-5793(90)81017-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using reversed micelles of surfactants solvated by water-organic cosolvent mixtures as a matrix for enzyme entrapping, it is possible to fix the highly reactive α-chymotrypsin form. The reactivity of α-chymotrypsin towards nonspecific substrates increases to the extent comparable with that observed in reactions involving specific substrates. © 1990.

引用

页码：238 / 240

页数：3

共 14 条

[11] MICELLAR ENZYMOLOGY [J].