ISOLATION AND CHARACTERIZATION OF A CDNA FOR RAT-LIVER CYSTEINE DIOXYGENASE

被引：42

作者：

HOSOKAWA, Y

MATSUMOTO, A

OKA, J

ITAKURA, H

YAMAGUCHI, K

机构：

[1] TOKYO KASEI UNIV,SCH HOME ECON,DEPT BIOCHEM,1-18-1 KAGA,KITA KU,TOKYO 173,JAPAN

[2] NATL INST HLTH & NUTR,SHINJUKU KU,TOKYO 162,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 168卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)92345-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cysteine dioxygenase is a key enzyme of cysteine metabolism in mammals. The cDNA clones for rat liver cysteine dioxygenase were isolated by immunological screening and plaque hybridization from a rat liver cDNA library. The longest clone contained an insert of 1458 bp and encoded a polypeptide of 200 amino acids. The clone included the corresponding nucleotide sequence to amino acid sequences obtained from four lysyl endopeptidase-digested fragments of purified rat liver cysteine dioxygenase. The calculated molecular weight of rat liver cysteine dioxygenase was 23,025. Northern blot analysis revealed a single cysteine dioxygenase mRNA species of about 1.7 kb. A computer homology search indicated that this protein showed no homology with any known protein. © 1990.

引用

页码：473 / 478

页数：6

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